Composite

Part:BBa_K2680124:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G pTlpAr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109


Design Notes

pTlpA sequence from Piraner et. al 2017.1 Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"TlpA is transcriptional repressor protein and thermosensor found in Salmonella typhimurium."2

References

1Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233

2Telarovic, I. (2017, October 24). Part:BBa_K2500003:Design. Retrieved October 14, 2018, from https://parts.igem.org/Part:BBa_K2500003:Design